Nikon FN1 (FLAME)

Overview

Nikon FN1 developed especially for electrophysiological research, features enhanced operability to facilitate experiments.

This equipment was funded by [BBSRC, 2020], please reference the funder and [BB/T017627/1], in your publication

The Nikon FN1 in the FLAME facility has been designed to allow maximum flexibility for different kind of imaging experiments coupled (or not) with the two other main components of the system: the BioCam Duplex MEA system and the Polygon 1000 patterned illuminator. Depending on the experimental design these components can be added, removed, or modified by the facility staff.

The system is run through Nikon’s versatile Elements-JOBS software package and incorporates wizards to allow the automated capture and analysis of both fluorescence and colour images. 

Imaging modes

• Multi point x,y (2D) with tiling
• Volume x,y,z (3D)
• Time lapse x,y,z,t (4D)
• Fluorescence
• DIC
• Brightfield imaging
• Live Cell imaging

Specification

Objectives

Magnification	NA	Coverslips	Other modes	Immersion
2	0.1			Air
4	0.2			Air
10	0.3		DIC	Water Dipping
16	0.8		DIC	Water Dipping
20	0.45			Air
40	0.8		DIC	Water Dipping
60	1		DIC	Water Dipping

 

 

 

 

 

 

 

 

 

 

Filters (epi)

Cube	Example fluorophores	Excitation	Dichroic	Emission	Part Number
Chrome 89402	DAPI/FITC/TRITC/Cy5	375-407 466-495 542-566 623-653	413 500 573 660	418-450 506-531 577-609 665-724
Chroma 89403	CFP/YFP/RFP/Cy7	422-450 496-518 565-589	458 524 598	462-486 528-552 708-752
Chroma 49053	DAPI	350-420	425	440-480
Chroma 49001	CFP	426-446	455	460-500
Chroma 49002	GFP/FITC/Cy2	450-490	495	500-550
Chroma 49003	YFP	490-510	515	520-550
Chroma 49004	Cy3/TRITC/	532-557	565	570-640
Chroma 49008	mCherry/Texas red	540-580	585	593-668
Chroma 49009	Cy5/AF647	625-655	660	665-715
Chroma 49007	Cy7/AF750	560-584	590	598-660
Chroma 79002	Fura 2/BCECF (ratiometric)	335-350 375-390 426-446 490-500	510	520-560

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Light source

CoolLed pE800  

CoolLed pE340

Location

FLAME facility, Ground floor Cookson Building, Medical School, IoN G.32 / 2.010

Medical School

Newcastle University

Newcastle Upon Tyne

NE2 4HH

SOP

Training Video

Booking